T7 RNA Polymerase GMP-grade (250 U/μL)-100 KU

This product is a T7 RNA polymerase enzyme derived from recombinant expression in Escherichia coli. It synthesizes RNA in the 5' to 3' direction using double-stranded DNA templates containing the T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and nucleotide triphosphates (NTPs) as substrates. The enzyme is compatible with linear DNA templates featuring blunt ends or 5' overhangs, making it suitable for in vitro RNA synthesis from linearized plasmids or PCR-amplified products.